Microbial production, immobilization and applications of β-D-galactosidase - Panesar, P. S.; Panesar, R.; Singh, R. S.; Kennedy, J. F.; Kumar, H. (J Chem Technol Biotechnol, 2006)

Technology prospecting on enzymes: application, marketing and engineering - Li, S.; Yang, X.; Yang, S.; Zhu, M.; Wang, X. (Comput Struct Biotechnol J, 2012)

Scotta F, Vera C, Conejeros R. Technical and economic analysis of industrial production of lactose-derived prebiotics with focus on galacto-oligosaccharides. In: Illanes A, Guerrero C, Vera C, Wilson L, Conejeros R, Scotta F, editors. Lactose-derived prebiotics: a process perspect. Elsevier Inc.; 2016. pp. 261–84. https://doi.org/10.1016/B978-0-12-802724-0.00007-X.

Fischer L, Lutz-Wahl S. Paenibacillus wynnii beta-galactosidase for the production of lactose-depleted dairy products. EP3682748A1. 2020.

Lutz-Wahl S, Mozer H, Kussler A, Schulz A, Seitl I, Fischer L. A new β-galactosidase from Paenibacillus wynnii with potential for industrial applications. J Dairy Sci. 2024;107:3429–42. https://doi.org/10.3168/jds.2023-24122.

Enzymes: principles and biotechnological applications - Robinson, P. K. (Essays Biochem, 2015)

Production of recombinant proteins by microbes and higher organisms - Demain, A. L.; Vaishnav, P. (Biotechnol Adv, 2009)

Quo vadis? The challenges of recombinant protein folding and secretion in Pichia pastoris - Puxbaum, V.; Mattanovich, D.; Gasser, B. (Appl Microbiol Biotechnol, 2015)

Protein secretion in gram-positive bacteria: from multiple pathways to biotechnology - Anné, J. M.; Economou, A.; Bernaerts, K. (Curr Top Microbiol Immunol, 2017)

Panel EFSABIOHAZ, Koutsoumanis K, Allende A, Alvarez-Ordonez A, Bolton D, Bover-Cid S, Chemaly M et al. The 2016 updated list of QPS status recommended biological agents in support of EFSA risk assessments. EFSA J. 2017;17(7). https://doi.org.10.5281/zenodo.1146566.

Developments in the use of Bacillus species for industrial production - Schallmey, M.; Singh, A.; Ward, O. P. (Can J Microbiol, 2004)

Recombinant protein secretion by Bacillus subtilis and Lactococcus lactis: pathways, applications, and innovation potential - Neef, J.; Dijl, J. M.; Buist, G. (Essays Biochem, 2021)

Proteomics of protein secretion by Bacillus subtilis: separating the secrets of the secretome - Tjalsma, H.; Antelmann, H.; Jongbloed, J. D. H.; Braun, P. G.; Darmon, E.; Dorenbos, R. (Microbiol Mol Biol Rev, 2004)

Functional genomic analysis of the Bacillus subtilis Tat pathway for protein secretion - Dijl, J. M.; Braun, P. G.; Robinson, C.; Quax, W. J.; Antelmann, H.; Hecker, M. (J Biotechnol, 2002)

Extracellular secretion in Bacillus subtilis of a cytoplasmic thermostable β-galactosidase from Geobacillus stearothermophilus - Xia, Y.; Zhao, J.; Chen, H.; Liu, X.; Wang, Y.; Tian, F. (J Dairy Sci, 2010)

Systematic screening of all signal peptides from Bacillus subtilis: a powerful strategy in optimizing heterologous protein secretion in Gram-positive bacteria - Brockmeier, U.; Caspers, M.; Freudl, R.; Jockwer, A.; Noll, T.; Eggert, T. (J Mol Biol, 2006)

Gerlach R, Pop O, Müller JP. Tat dependent export of E. coli phytase AppA by using the PhoD-specific transport system of Bacillus subtilis. J Basic Microbiol. 2004;44:351–9. https://doi.org/10.1002/jobm.200410423.

Promoter screening from Bacillus subtilis in various conditions hunting for synthetic biology and industrial applications - Song, Y.; Nikoloff, J. M.; Fu, G.; Chen, J.; Li, Q.; Xie, N. (PLoS ONE, 2016)

Zhou C, Ye B, Cheng S, Zhao L, Liu Y, Jiang J, et al. Promoter engineering enables overproduction of foreign proteins from a single copy expression cassette in Bacillus subtilis. Microb Cell Fact. 2019;111. https://doi.org/10.1186/s12934-019-1159-0.

Systematic screening of optimal signal peptides for secretory production of heterologous proteins in Bacillus subtilis - Fu, G.; Liu, J.; Li, J.; Zhu, B.; Zhang, D. (J Agric Food Chem, 2018)

Fed-batch biomolecule production by Bacillus subtilis: a state of the art review - Öztürk, S.; Çalik, P.; Özdamar, T. H. (Trends Biotechnol, 2016)

Enhanced secretory production of a single-chain antibody fragment from Bacillus subtilis by coproduction of molecular chaperones - Wu, S. C.; Ye, R.; Wu, X. C.; NG, S. C.; Wong, S. L. (J Bacteriol, 1998)

Engineering a Bacillus subtilis expression-secretion system with a strain deficient in six extracellular proteases - Wu, X. C.; Lee, W.; Tran, L.; Wong, S. L. (J Bacteriol, 1991)

Optimization of protein secretion by Bacillus subtilis - Nijland, R.; Kuipers, O. (Recent Pat Biotechnol, 2008)

Evaluation of bottlenecks in the late stages of protein secretion in Bacillus subtilis - Bolhuis, A.; Tjalsma, H.; Smith, H. E.; Jong, A.; Meima, R.; Venema, G. (Appl Environ Microbiol, 1999)

Bottlenecks in the expression and secretion of heterologous proteins in Bacillus subtilis - Li, W.; Zhou, X.; Lu, P. (Res Microbiol, 2004)

Construction of inducible secretion vectors and their application for the secretion of foreign extracellular and intracellular proteins in Bacillus subtilis - Imanaka, T.; Takagi, M.; Shima, H.; Bhatnagar, L.; Gregory Zeikus, J. (J Ferment Bioeng, 1993)

Efficient secretory production of large-size heterologous enzymes in Bacillus subtilis: a secretory partner and directed evolution - Liu, S.; Wang, J.; Zhu, Z.; Shi, T.; Zhang, Y. H. P. J. (Biotechnol Bioeng, 2020)

β-Galactosidase BMG without galactose and glucose inhibition: secretory expression in Bacillus subtilis and for synthesis of oligosaccharide - Zhao, L.; Zhou, Y.; Qin, S.; Qin, P.; Chu, J.; He, B. (Int J Biol Macromol, 2018)

High-expression keratinase by Bacillus subtilis SCK6 for enzymatic dehairing of goatskins - Tian, J.; Xu, Z.; Long, X.; Tian, Y.; Shi, B. (Int J Biol Macromol, 2019)

Enhanced and secretory expression of human granulocyte colony stimulating factor by Bacillus subtilis SCK6 - Bashir, S.; Sadaf, S.; Ahmad, S.; Akhtar, M. W. (Biomed Res Int, 2015)

Cloning, heterologous expression and characterization of a novel streptomyces trypsin in Bacillus subtilis SCK6 - Wang, Z.; Li, X.; Tian, J.; Chu, Y.; Tian, Y. (Int J Biol Macromol, 2020)

Heterologous expression of recombinant transglutaminase in Bacillus subtilis SCK6 with optimized signal peptide and codon, and its impact on gelatin properties - Wang, S.; Yang, Z.; Li, Z.; Tian, Y. (J Microbiol Biotechnol, 2020)

Construction of a Bacillus subtilis double mutant deficient in extracellular alkaline and neutral proteases - Kawamura, F.; Doi, R. H. (J Bacteriol, 1984)

Simple, fast and high-efficiency transformation system for directed evolution of cellulase in Bacillus subtilis - Zhang, X. Z.; Zhang, Y. H. P. (Microb Biotechnol, 2011)

High-level expression and secretion of methyl parathion hydrolase in Bacillus subtilis WB800 - Zhang, X. Z.; Cui, Z. L.; Hong, Q.; Li, S. P. (Appl Environ Microbiol, 2005)

Optimization of staphylokinase production in Bacillus subtilis using inducible and constitutive promoters - Kim, J. H.; Wong, S. L.; Kim, B. G. (Biotechnol Bioprocess Eng, 2001)

Improvement of culture conditions to overproduce β-galactosidase from Escherichia coli in Bacillus subtilis - Martínez, A.; Ramírez, O. T.; Valle, F. (Appl Microbiol Biotechnol, 1997)

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding - Bradford, M. M. (Anal Biochem, 1976)

Novel high-performance metagenome β-galactosidases for lactose hydrolysis in the dairy industry - Erich, S.; Kuschel, B.; Schwarz, T.; Ewert, J.; Böhmer, N.; Niehaus, F. (J Biotechnol, 2015)

Cleavage of structural proteins during the assembly of the head of bacteriophage T4 - Laemmli, U. K. (Nature, 1970)

Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane - Fairbanks, G.; Steck, T. L.; Wallach, D. F. H. (Biochem, 1971)

Mass spectrometric sequencing of proteins from silver-stained polyacrylamide gels - Shevchenko, A.; Wilm, M.; Vorm, O.; Mann, M. (Anal Chem, 1996)

Automated design of synthetic ribosome binding sites to control protein expression - Salis, H. M.; Mirsky, E. A.; Voigt, C. A. (Nat Biotechnol, 2009)

Characterization of the 5’ subtilisin (aprE) regulatory region from Bacillus subtilis - Jan, J.; Valle, F.; Bolivar, F.; Merino, E. (FEMS Microbiol Lett, 2000)

Overlapping promoters transcribed by Bacillus subtilis σ55 and σ37 RNA polymerase holoenzymes during growth and stationary phases - Wang, P. Z.; Doi, R. H. (J Biol Chem, 1984)

Genetic system constructed to overproduce and secrete proinsulin in Bacillus subtilis - Olmos-Soto, J.; Contreras-Flores, R. (Appl Microbiol Biotechnol, 2003)

Enhancement of secretion and extracellular stability of staphylokinase in Bacillus subtilis by wprA gene disruption - Lee, S. J.; Kim, D. M.; Bae, K. H.; Byun, S. M.; Chung, J. H. (Appl Environ Microbiol, 2000)

Efficient secretion of the β-galactosidase Bgal1-3 via both Tat-dependent and Tat-independent pathways in Bacillus subtilis - Ren, G. H.; Cao, L. C.; Kong, W.; Wang, Z. J.; Liu, Y. H. (J Agric Food Chem, 2016)

Twin-arginine signal peptide of Bacillus licheniformis GlmU efficiently mediated secretory expression of protein glutaminase - Niu, D.; Li, C.; Wang, P.; Huang, L.; Mchunu, N. P.; Singh, S. (Electron J Biotechnol, 2019)

The twin-arginine signal peptide of Bacillus subtilis YwbN can direct either Tat- or sec-dependent secretion of different cargo proteins: secretion of active subtilisin via the B. subtilis Tat pathway - Kolkman, M. A. B.; Ploeg, R.; Bertels, M.; Dijk, M.; Laan, J.; Dijl, J. M. (Appl Environ Microbiol, 2008)

Markedly unbiased codon usage in Bacillus subtilis - Naotake, O. (Gene, 1985)

Codon usage and lateral gene transfer in Bacillus subtilis - Moszer, I.; Rocha, E. P. C.; Danchin, A. (Curr Opin Microbiol, 1999)

Signal peptides for recombinant protein secretion in bacterial expression systems - Freudl, R. (Microb Cell Fact, 2018)

Signal peptide-dependent protein transport in Bacillus subtilis: a genome-based survey of the secretome - Tjalsma, H.; Bolhuis, A.; Jongbloed, J. D. H.; Bron, S.; Dijl, J. M. (Microbiol Mol Biol Rev, 2000)

Enhancement of pullulanase production from recombinant Bacillus subtilis by optimization of feeding strategy and fermentation conditions - Zhang, Y.; Nie, Y.; Zhou, X.; Bi, J.; Xu, Y. (AMB Express, 2020)

Metabolic engineering of Bacillus subtilis for high-titer production of menaquinone-7 - Yang, S.; Wang, Y.; Cai, Z.; Zhang, G.; Song, H. (AIChE J, 2020)

Hyperphosphorylation of DegU cancels CcpA-dependent catabolite repression of rocG in Bacillus subtilis - Tanaka, K.; Iwasaki, K.; Morimoto, T.; Matsuse, T.; Hasunuma, T.; Takenaka, S. (BMC Microbiol, 2015)

Nicaud JM, Madzak C, Van Den Broek P, Gysler C, Duboc P, Niederberger P, et al. Protein expression and secretion in the yeast Yarrowia lipolytica. FEMS Yeast Res. 2002;2:371–9. https://doi.org/10.1016/S1567-1356(02)00082-X.

Protein secretion in Pichia pastoris and advances in protein production - Damasceno, L. M.; Huang, C. J.; Batt, C. A. (Appl Microbiol Biotechnol, 2012)

Characterization and application of endogenous phase-dependent promoters in Bacillus subtilis - Yang, S.; Du, G.; Chen, J.; Kang, Z. (Appl Microbiol Biotechnol, 2017)

Enhanced expression of pullulanase in Bacillus subtilis by new strong promoters mined from transcriptome data, both alone and in combination - Meng, F.; Zhu, X.; Nie, T.; Lu, F.; Bie, X.; Lu, Y. (Front Microbiol, 2018)

Trehalose production using recombinant trehalose synthase in Bacillus subtilis by integrating fermentation and biocatalysis - Liu, H.; Wang, S.; Song, L.; Yuan, H.; Liu, K.; Meng, W. (J Agric Food Chem, 2019)

Comparison of PaprE, PamyE, and PP43 promoter strength for β-galactosidase and staphylokinase expression in Bacillus subtilis - Kim, J. H.; Hwang, B. Y.; Roh, J.; Lee, J. K.; Kim, K.; Wong, S. L. (Biotechnol Bioprocess Eng, 2008)

The aprE leader is a determinant of extreme mRNA stability in Bacillus subtilis - Hambraeus, G.; Persson, M.; Rutberg, B. (Microbiology, 2000)

Influence of promoter and signal peptide on the expression of pullulanase in Bacillus subtilis - Wang, Y.; Liu, Y.; Wang, Z.; Lu, F. (Biotechnol Lett, 2014)

Rational design of the N-terminal coding sequence for regulating enzyme expression in Bacillus subtilis - Xu, K.; Tong, Y.; Li, Y.; Tao, J.; Li, J.; Zhou, J.; Liu, S. (ACS Synth Biol, 2021)

The length of ribosomal binding site spacer sequence controls the production yield for intracellular and secreted proteins by Bacillus subtilis - Volkenborn, K.; Kuschmierz, L.; Benz, N.; Lenz, P.; Knapp, A.; Jaeger, K. E. (Microb Cell Fact, 2020)

Efficient translation initiation dictates codon usage at gene start - Bentele, K.; Saffert, P.; Rauscher, R.; Ignatova, Z.; Blüthgen, N. (Mol Syst Biol, 2013)